The CD44 Antibody: Learn More About It

The CD44 antibody is a cell-surface glycoprotein and can be found in a variety of blood cells. It is also considered a homing cell adhesion molecule on EMC-III, HUTCH-1, Hermes-1, and Phagocytic glycoprotein-1. It has also been implicated in lymphocyte homing, tumor metastasis, and cell migration.

More Information

Do not use the CD44 for diagnostic procedures. It does have a clone called SPM 521, and the immunogen is the synthetic peptide that corresponds to the human CD44. The isotype is the IgG2a, and it has an undetermined epitope. The molecular weight is 80kDa.

Applications

The CD44 antibody can be used in Immunohistochemistry and Western Blotting applications.

The IHC procedure includes preparing the specimen using a Formalin-fixed or paraffin-embedded tissue. Deparaffinized slides should be used, as well. When choosing the concentrated format, you’ll want to dilute the antibody using a ratio of 1:50, though dilutions are estimates. Your protocol may require a different dilution. You can also find a pre-diluted formation that will work for IHC applications only.

To retrieve the antigen, you’ll want to boil the tissue section in a 10mM Citrate buffer with a pH of 6.0 for at least 10 minutes, giving 20 minutes to cool to room temperature. Incubate the antibody for 30 minutes while at room temperature.

The positive control is the Esophageal carcinoma with cellular localization occurring in the membrane.

For western Blotting applications, you’ll want to dilute the antibody using a ratio of 1:25, which means you cannot use the pre-diluted formation that is available. Incubate it for one hour at room temperature. The positive control for Western Blotting is the HeLa cell lysate.

The CD44 antibody can be used in two applications and can be used to test various things. Visit Spring Bioscience today to learn more now.

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